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dreamlet

Bioc current

Scalable differential expression analysis of single cell transcriptomics datasets with complex study designs

v1.10.0 · software · Artistic-2.0

Release Lineage

Entered 3.18 · Oct 25, 2023

Current · Requires R 4.6

1.0 In 6 of 49 releases 3.23

Description

Recent advances in single cell/nucleus transcriptomic technology has enabled collection of cohort-scale datasets to study cell type specific gene expression differences associated disease state, stimulus, and genetic regulation. The scale of these data, complex study designs, and low read count per cell mean that characterizing cell type specific molecular mechanisms requires a user-frieldly, purpose-build analytical framework. We have developed the dreamlet package that applies a pseudobulk approach and fits a regression model for each gene and cell cluster to test differential expression across individuals associated with a trait of interest. Use of precision-weighted linear mixed models enables accounting for repeated measures study designs, high dimensional batch effects, and varying sequencing depth or observed cells per biosample.

Test coverage

Line coverage

Expression

Tests / Examples

Functions

57 22 exported

Complexity

4.9 avg / 43 max

Call network

57 nodes / 45 edges

Test coverage is not measured for Bioconductor packages; nodes fall back to a neutral fill.

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Lowest coverage

Per-function coverage is not measured for this package yet.

Code

Structure

Lines of code

12,596

Files

429

Compiled share

1%

Has compiled src

Yes

Language breakdown

R 7,008 (55.6%)C/C++/src 122 (1%)Tests 8 (0.1%)Docs 3,344 (26.5%)Vignettes 2,114 (16.8%)

API

Exported functions

35

Internal functions

30

Recent export changes

v3.20−2 getExprGeneNames, pbWeights

Testing & CI

Has tests

Yes

Test-to-code ratio

0.00

testthat edition

CI present

Yes

CI type

["github-actions"]

PR gated

No

Docs

Roxygen coverage

100%

Health & Security signals

Informational signals; not verdicts.

on.exit coverage

Unsafe pattern score

0

Dep constraint coverage

7.1%

Secret pattern count

0

Bundled 3rd-party code

2 items

Portability & License

Min R version

4.3.0

System requirements

1

C++ standard

License

Artistic-2.0

License flags

SPDX valid, OSI approved

History

Versions

6

First release

2024-02-27

Latest release

2026-04-28

Avg cadence

161 days

Cold removal rate

100%

Dep drift

4

LOC over versions

v3.18: 19,795 LOCv3.19: 19,795 LOCv3.20: 19,214 LOCv3.21: 19,233 LOCv3.22: 19,234 LOCv3.23: 12,596 LOC

Per-file churn detail lives in the source pipeline: https://github.com/r-observatory/bioc-code-metrics.

Documentation

Documentation
READMEYes · 454 wordsVignettesYes · dynamicpkgdown siteYesNEWSYes · 67% structuredCode of conductNoContributing guideNo
Examples that run
100%
Documented parameters
95%
Return-value docs
94%
References docs
5%

Topics

Depended on by (1)

Bioconductor (1)

People

Cite

Cite this package

Run in R for the authors' preferred citation:

citation("dreamlet")
Hoffman, G. (2026). dreamlet: Scalable differential expression analysis of single cell transcriptomics datasets with complex study designs (Version 1.10.0) [Computer software]. https://bioconductor.org/packages/dreamlet

This is what citation() produces when a package has no citation file of its own. If it prints something else, use that.

Cite the R Observatory

For a number measured here: a download total, a coverage figure, an archival date.

APA

Balamuta, J. J. (2026). R Observatory: Metrics for dreamlet version 1.10.0 [Data set]. HJJB, LLC. Data release v2026-08-23. https://doi.org/10.5281/zenodo.21843040

From data release v2026-08-23, which the citation names so these numbers can be found later. More on citing and the projects behind them.

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