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stPipe

Bioc current

Upstream pre-processing for Sequencing-Based Spatial Transcriptomics

v1.2.0 · Package record · software · GPL-3

Release Lineage

Entered 3.22 · Oct 30, 2025

Current · Requires R 4.6

1.0 In 2 of 49 releases 3.23

Description

This package serves as an upstream pipeline for pre-processing sequencing-based spatial transcriptomics data. Functions includes FASTQ trimming, BAM file reformatting, index building, spatial barcode detection, demultiplexing, gene count matrix generation with UMI deduplication, QC, and revelant visualization. Config is an essential input for most of the functions which aims to improve reproducibility.

Advisories

None published for Bioconductor

Test coverage

Line coverage

–

Expression

–

Tests / Examples

–

Functions

27 8 exported

Complexity

4.6 avg / 20 max

Call network

27 nodes / 4 edges

Test coverage is not measured for Bioconductor packages; nodes fall back to a neutral fill.

Loading call graph…

Lowest coverage

Per-function coverage is not measured for this package yet.

Code

Structure

Lines of code

3,002

Files

57

Compiled share

10.4%

Has compiled src

Yes

Language breakdown

R 1,694 (56.4%)C/C++/src 313 (10.4%)Tests 283 (9.4%)Docs 496 (16.5%)Vignettes 216 (7.2%)

API

Exported functions

8

Internal functions

9

Recent export changes

v3.22+8 Run_Clustering, Run_Create_Obj, Run_HTML +5 more

Testing

Has tests

Yes

Test-to-code ratio

0.17

testthat edition

3

Docs

Roxygen coverage

100%

Health & Security signals

Informational signals; not verdicts.

on.exit coverage

0%

Unsafe pattern score

6

Dep constraint coverage

0%

Secret pattern count

0

Bundled 3rd-party code

2 items

Portability & License

Min R version

4.5.0

System requirements

1 externalGNU make

C++ standard

–

License

GPL-3

License flags

SPDX valid, OSI approved

History

Versions

2

First release

2025-11-20

Latest release

2026-04-28

Avg cadence

159 days

Cold removal rate

–

Dep drift

0

LOC over versions

v3.22: 3,002 LOCv3.23: 3,002 LOC

Per-file churn detail lives in the source pipeline: https://github.com/r-observatory/bioc-code-metrics.

Documentation

In the Bioconductor 3.23 release
READMEYes · 89 wordsVignettesYes · 1 · dynamicNEWSYesCitation fileYes · cites a publicationWebsitenot declared in DESCRIPTION
Help pages
9includes a package overview page
Examples that run
100%7 run in a plain check
Exported functions with examples
88%
Documented parameters
100%
Return-value docs
100%
Help pages citing references
0%

Repository practices

Upstream repositoryBeta

1 development-tooling and community-health practice detected across 1 family in the upstream repository

Checks run against github.com/mritchielab/stpipe on 2026-10-18.

Lint, format, editor (1)
RStudio project
How this is detected·Detection ruleset v3 (2026-09-26)

Datasets

Bundled datasets · 9
NameClassSizeAlso in
Visium_Human_Transcriptome_Probe_Set_v1_0_GRCh38_2020_Adata.frame18,681 × 2No other package
Visium_Human_Transcriptome_Probe_Set_v2_0_GRCh38_2020_Adata.frame53,504 × 2No other package
Visium_Mouse_Transcriptome_Probe_Set_v1_0_mm10_2020_Adata.frame20,057 × 2No other package
ref_posdata.frame4,991 × 6No other package
visium_v1_coordinatesdata.frame4,992 × 3No other package
visium_v2_coordinatesdata.frame4,992 × 3No other package
visium_v3_coordinatesdata.frame4,992 × 3No other package
visium_v4_coordinatesdata.frame4,992 × 3No other package
visium_v5_coordinatesdata.frame14,336 × 3No other package

All of stPipe's data objects

Topics

Maintainer
Yang Xu

Available on

CRANr2uBioconductorautoCRANCOPR

People

Cite

Cite this package

Run in R for the authors' entry:

citation("stPipe")

The package provides a citation file, so citation() returns the authors' own entry.

It points to 10.1093/nargab/lqaf167.

Cite the R Observatory

For a number measured here: a download total, a coverage figure, an archival date.

APA

Balamuta, J. J. (2026). R Observatory: Metrics for stPipe version 1.2.0 [Data set]. HJJB, LLC. Data release v2026-10-22. https://doi.org/10.5281/zenodo.21843040

From data release v2026-10-22, which the citation names so these numbers can be found later. More on citing and the projects behind them.

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