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GUIDEseq

Bioc current

GUIDE-seq and PEtag-seq analysis pipeline

v1.42.0 · Package record · software · GPL (>= 2)

Release Lineage

Entered 3.2 · Oct 14, 2015

Current · Requires R 4.6

1.0 In 22 of 49 releases 3.23

Description

The package implements GUIDE-seq and PEtag-seq analysis workflow including functions for filtering UMI and reads with low coverage, obtaining unique insertion sites (proxy of cleavage sites), estimating the locations of the insertion sites, aka, peaks, merging estimated insertion sites from plus and minus strand, and performing off target search of the extended regions around insertion sites with mismatches and indels.

Advisories

None published for Bioconductor

Test coverage

Line coverage

–

Expression

–

Tests / Examples

–

Functions

38 15 exported

Complexity

9.6 avg / 52 max

Call network

38 nodes / 25 edges

Test coverage is not measured for Bioconductor packages; nodes fall back to a neutral fill.

Loading call graph…

Lowest coverage

Per-function coverage is not measured for this package yet.

Code

Structure

Lines of code

9,953

Files

99

Compiled share

0%

Has compiled src

No

Language breakdown

R 5,998 (60.3%)Tests 1,245 (12.5%)Docs 2,433 (24.4%)Vignettes 277 (2.8%)

API

Exported functions

15

Internal functions

19

Recent export changes

v3.5+2 createBarcodeFasta, getUsedBarcodes

Testing

Has tests

Yes

Test-to-code ratio

0.21

testthat edition

3

Docs

Roxygen coverage

100%

Health & Security signals

Informational signals; not verdicts.

on.exit coverage

–

Unsafe pattern score

0

Dep constraint coverage

10%

Secret pattern count

0

Bundled 3rd-party code

2 items

Portability & License

Min R version

3.5.0

System requirements

–

C++ standard

–

License

GPL (>= 2)

License flags

SPDX valid, OSI approved

History

Versions

22

First release

2015-10-28

Latest release

2026-04-28

Avg cadence

182 days

Cold removal rate

–

Dep drift

20

LOC over versions

v3.2: 2,131 LOCv3.3: 2,807 LOCv3.4: 3,241 LOCv3.5: 3,488 LOCv3.6: 3,542 LOCv3.7: 3,544 LOCv3.8: 3,544 LOCv3.9: 3,544 LOCv3.10: 3,544 LOCv3.11: 3,602 LOCv3.12: 3,602 LOCv3.13: 3,602 LOCv3.14: 3,602 LOCv3.15: 3,944 LOCv3.16: 8,985 LOCv3.17: 9,951 LOCv3.18: 9,951 LOCv3.19: 9,953 LOCv3.20: 9,953 LOCv3.21: 9,953 LOCv3.22: 9,953 LOCv3.23: 9,953 LOC

Per-file churn detail lives in the source pipeline: https://github.com/r-observatory/bioc-code-metrics.

Documentation

In the Bioconductor 3.23 release
READMENoVignettesYes · 1 · dynamicNEWSYes · 0% structuredCitation fileYes · cites a publicationWebsitenot declared in DESCRIPTION
Help pages
20includes a package overview page
Examples that run
100%18 run in a plain check (12 of them under a condition)
Exported functions with examples
93%
Documented parameters
91%
Return-value docs
80%
Help pages citing references
45%
Vignettes (1)

Repository practices

Bioconductor git repository

Checks read the Bioconductor git repository (git.bioconductor.org), branch RELEASE_3_23.

No development-tooling practices detected in the Bioconductor git repository.

Datasets

Bundled datasets · 18
NameClassSizeAlso in
1450peakSeq–––
InsertionInOfftargetlist3 elementsNo other package
PEtagAnalysis_testthatlist6 elementsNo other package
UMI-HEK293_site4_R1data.frame5,571 × 3No other package
UMI-HEK293_site4_chr13data.frame30,096 × 3No other package
alnsdata.frame2 × 212 packages
both.runsum–––
cleavages_testthatlist7 elementsNo other package
consecutiveDNABulgedata.frame1 × 19No other package
consecutiveDNABulge2data.frame1 × 19No other package
mergedOffTargetsdata.frame109 × 30No other package
pa.flist123 elementsNo other package
pa.rlist123 elementsNo other package
peaks–––
peaks.gr–––
subjects2–––
testCFDscoringWithBulgedata.frame2 × 29No other package
uniqueCleavageEventslist6 elementsNo other package

All of GUIDEseq's data objects

Topics

Maintainer
Lihua Julie Zhu

Available on

CRANr2uBioconductorautoCRANCOPR

Depended on by (1)

Bioconductor (1)

People (from the free-text Author field)

Cite

Cite this package

Run in R for the authors' entry:

citation("GUIDEseq")

The package provides a citation file, so citation() returns the authors' own entry.

Cite the R Observatory

For a number measured here: a download total, a coverage figure, an archival date.

APA

Balamuta, J. J. (2026). R Observatory: Metrics for GUIDEseq version 1.42.0 [Data set]. HJJB, LLC. Data release v2026-10-20. https://doi.org/10.5281/zenodo.21843040

From data release v2026-10-20, which the citation names so these numbers can be found later. More on citing and the projects behind them.

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