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DOTSeq

1.0.0Bioconductor

Genome-wide Detection of Differential ORF Usage

BiocManager::install("DOTSeq")
Distinct IPs a month, method
371Rank 2,342 in its category
Bioconductor releases
1since 2026
Advisories, method
None publishedfor Bioconductor

Differential open reading frame (ORF) translation analysis framework for ribosome profiling (Ribo-seq) with matched RNA-seq.

Full description

Differential open reading frame (ORF) translation analysis framework for ribosome profiling (Ribo-seq) with matched RNA-seq. Implements (i) Differential ORF Usage (DOU), a beta-binomial generalized linear model that models the expected proportion of Ribo-seq versus RNA-seq reads mapping to each ORF within a gene, and (ii) ORF-level Differential Translation Efficiency (DTE), a negative binomial GLM that capture changes in translation efficiency of individual ORFs across experimental conditions. Supports ORF-level read summarization for bulk and single-cell Ribo-seq.

Maintainer
Chun Shen Lim
Last updated
28 Apr 2026
First release
Bioconductor 3.23, 29 Apr 2026

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More details
Releases
1 of 49 Bioconductor releases
Category
software
RoxygenNote
7.3.3
biocViews (12)
Cite

Package

Run in R for the authors' entry:

citation("DOTSeq")

The package provides a citation file, so citation() returns the authors' own entry.

It points to 10.1101/2025.09.24.678201.

R Observatory

For a number measured here: a download total, a coverage figure, an archival date.

APA
Balamuta, J. J. (2026). R Observatory: Metrics for DOTSeq version 1.0.0 [Data set]. HJJB, LLC. Data release v2026-10-31. https://doi.org/10.5281/zenodo.21843040
About

From data release v2026-10-31, which the citation names so these numbers can be found later.

Release 3.23: OK 29 Oct 2026

  • kunpeng2, Install

    Install on the build machine kunpeng2. Bioconductor release 3.23.

    OK

    Version built
    1.0.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • kunpeng2, Build

    Build on the build machine kunpeng2. Bioconductor release 3.23.

    OK

    Version built
    1.0.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • kunpeng2, Check

    Check on the build machine kunpeng2. Bioconductor release 3.23.

    OK

    Version built
    1.0.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • nebbiolo1, Install

    Install on the build machine nebbiolo1. Bioconductor release 3.23.

    OK

    Version built
    1.0.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • nebbiolo1, Build

    Build on the build machine nebbiolo1. Bioconductor release 3.23.

    OK

    Version built
    1.0.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • nebbiolo1, Check

    Check on the build machine nebbiolo1. Bioconductor release 3.23.

    OK

    Version built
    1.0.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • 6 OK
Machines (2)
MachineInstallBuildCheck
kunpeng2OKOKOK
nebbiolo1OKOKOK
Devel 3.24: OK
  • lconway, Install

    Install on the build machine lconway. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    28 Oct 2026
  • lconway, Build

    Build on the build machine lconway. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    28 Oct 2026
  • lconway, Check

    Check on the build machine lconway. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    28 Oct 2026
  • lconway, Build binary

    Build binary on the build machine lconway. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    28 Oct 2026
  • nebbiolo2, Install

    Install on the build machine nebbiolo2. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • nebbiolo2, Build

    Build on the build machine nebbiolo2. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • nebbiolo2, Check

    Check on the build machine nebbiolo2. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • taishan, Install

    Install on the build machine taishan. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • taishan, Build

    Build on the build machine taishan. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • taishan, Check

    Check on the build machine taishan. The next Bioconductor release, 3.24.

    OK

    Version built
    1.1.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    29 Oct 2026
  • 10 OK
MachineInstallBuildCheckBuild binary
lconwayOKOKOKOK
nebbiolo2OKOKOK
taishanOKOKOK
Built files (4)
PlatformKindFile
macOS (Intel)binaryDOTSeq_1.0.0.tgz
macOS (Apple Silicon)binaryDOTSeq_1.0.0.tgz
sourcesourceDOTSeq_1.0.0.tar.gz
WindowsbinaryDOTSeq_1.0.0.zip
Total downloads in the latest complete month
2,404It comes from Bioconductor alone. Bioconda was counted over another window, so it is not added.

Rank in category, by distinct IPs

2,342 of 3,173

Bioconductor

2,404 downloads in the latest complete month

Distinct IP addresses average 371 a month over 12 months, which is a different measure and is never added.

It is in the total. Take it out

Bioconda

11 downloads in the last 30 days

It is not added because it has no figure for the latest complete month.

This local copy has no series over time.

Compare downloads

Functions
81
Public exports
24
Lines of R code
9,719
Median complexity
4
Functions (81)
Size and composition

Lines by kind (17,726)

KindLinesShare
R code9,71955%
Tests1,87311%
Documentation4,61126%
Guides1,0696%
Compiled code4543%

Lines by language

LanguageLinesShare
R11,59265%
Rd4,61126%
Vignettes6574%
C++4423%
Other4122%
C120.1%

Languages are counted from file extensions.

Files
123
R functions
67
Compiled functions
14
Object systems
S4: 4 classes, S4: 11 generics, S4: 13 methods
Complexity
Median
4
Mean
8.84
Highest
78
Median lines per function
41
About complexity

Cyclomatic complexity counts the independent paths through a function: 1 is a straight line, higher is harder to test. R functions only.

Call graph figures
R functions
67
Calls
77
Unconnected
4
Groups
1

Test coverage is measured for CRAN packages only. Nothing is known for Bioconductor packages, which is not the same as no coverage.

Test cases
62
Lines of tests
1,873
Exports named in tests
79.2%
Has tests
Yes
Test frameworks
testthat
Test lines per line of code
0.19
Exports documented
100%
Arguments documented
89.9%
Return value documented
94.4%
Help pages with examples
49.3%
Examples run in checks
58.3%
Guides (1)
Other documentation
README
Yes, 369 words
Release notes file
Yes
Help pages
73
Lines of documentation
4,611

2 datasets, 0 can be loaded by name

DatasetSizeColumns
featureCounts.cell_cycle_subset6,642 × 1Geneid.Chr.Start.End.Strand.Length.ly_2024.ribo.chx.SRR24230465Aligned.sortedByCoord.out.bam.ly_2024.ribo.chx.SRR24230467Aligned.sortedByCoord.out.bam.ly_2024.ribo.chx.SRR24230469Aligned.sortedByCoord.out.bam.ly_2024.ribo.chx.SRR24230471Aligned.sortedByCoord.out.bam.ly_2024.ribo.chx.SRR24230480Aligned.sortedByCoord.out.bam.ly_2024.ribo.chx.SRR24230482Aligned.sortedByCoord.out.bam.ly_2024.rna.chx.SRR24230462Aligned.sortedByCoord.out.bam.ly_2024.rna.chx.SRR24230466Aligned.sortedByCoord.out.bam.ly_2024.rna.chx.SRR24230472Aligned.sortedByCoord.out.bam.ly_2024.rna.chx.SRR24230474Aligned.sortedByCoord.out.bam.ly_2024.rna.chx.SRR24230477Aligned.sortedByCoord.out.bam.ly_2024.rna.chx.SRR24230479Aligned.sortedByCoord.out.bam
metadata23 × 5SRR24230462, rna, X2, chx, Mitotic_Cycling

Funded by Marsden

compgenom / dotseq on github.com

Stars
1
Forks
0
Open issues
0
Last commit
31 Mar 2026
Activity
Commits
254
Open pull requests
0
Tagged releases
0
Latest tagged release
–
Issues closed in
–
Pull requests closed in
–
Pull requests merged
–
Created
16 Aug 2025
Licence file reads as
–

Bioconductor git

R releases
  • In Bioconductor
  • Bioconductor release
ReleaseReleasedR
3.23 current29 Apr 20264.6
Entered Bioconductor
Release 3.23, 29 Apr 2026