Visualising Splicing at Single-Cell Resolution
Overview
Alternative splicing produces a variety of different protein products from a given gene. 'VALERIE' enables visualisation of alternative splicing events from high-throughput single-cell RNA-sequencing experiments. 'VALERIE' computes percent spliced-in (PSI) values for user-specified genomic coordinates corresponding to alternative splicing events. PSI is the proportion of sequencing reads supporting the included exon/intron as defined by Shiozawa (2018) doi:10.1038/s41467-018-06063-x. PSI are inferred from sequencing reads data based on specialised infrastructures for representing and computing annotated genomic ranges by Lawrence (2013) doi:10.1371/journal.pcbi.1003118. Computed PSI for each single cell are subsequently presented in the form of a heatmap implemented using the 'pheatmap' package by Kolde (2010) https://CRAN.R-project.org/package=pheatmap. Board overview of the mean PSI difference and associated p-values across different user-defined groups of single cells are presented in the form of a line graph using the 'ggplot2' package by Wickham (2007) https://CRAN.R-project.org/package=ggplot2.
Install
Health
NOTECRAN incoming feasibilityr-devel-linux-x86_64-debian-clang, r-devel-linux-x86_64-debian-gcc
Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’
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Documentation
- Help pages
- 12
- Examples that run
- 100%12 run in a plain check
- Exported functions with examples
- 100%
- Documented parameters
- 88%
- Return-value docs
- 100%
- Help pages citing references
- 0%
- VALERIE PDF
Downloads
Dependencies
Installing VALERIE adds 76 packages (37 with compiled code, 67 MB of source tarballs). Heaviest: stringi 11 MB, ggplot2 6.0 MB, doBy 4.3 MB. 4 more not on CRAN are not measured.
Code & Tests
People & History
Pick two releases to compare their code metrics.
- RR 4.6.0 released · 2026-04-24
- RR 4.5.0 released · 2025-04-11
- RR 4.4.0 released · 2024-04-24
- RR 4.3.0 released · 2023-04-21
- RR 4.2.0 released · 2022-04-22
- RR 4.1.0 released · 2021-05-18
- 1.1.0Latest
- RR 4.0.0 released · 2020-04-24
- 1.0.02020-02-11
- RR 3.6.0 released · 2019-04-26
Package metadata
- First published
- 2020-02-11
- Total releases
- 2 / 6 yrs
- License
- GPL-3 OSI
- Download size
- 4.3 MB
Cite
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