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spiky

1.18.0Bioconductor

Spike-in calibration for cell-free MeDIP

Record of Bioconductor 3.23Compare with 3.22LicencesSBOM

BiocManager::install("spiky")
Distinct IPs a month, method
514Rank 1,824 in its category
Bioconductor releases
10since 2021
Advisories, method
None publishedfor Bioconductor

spiky implements methods and model generation for cfMeDIP (cell-free methylated DNA immunoprecipitation) with spike-in controls.

Full description

spiky implements methods and model generation for cfMeDIP (cell-free methylated DNA immunoprecipitation) with spike-in controls. CfMeDIP is an enrichment protocol which avoids destructive conversion of scarce template, making it ideal as a "liquid biopsy," but creating certain challenges in comparing results across specimens, subjects, and experiments. The use of synthetic spike-in standard oligos allows diagnostics performed with cfMeDIP to quantitatively compare samples across subjects, experiments, and time points in both relative and absolute terms.

Maintainer
Tim Triche
Licence
GPL-2
Last updated
28 Apr 2026
First release
Bioconductor 3.14, 27 Oct 2021

Compare with another package

More details
Releases
10 of 49 Bioconductor releases
Category
software
Needs R
>= 3.6.0
Date
2023-04-19
LazyData
true
RoxygenNote
7.2.1
biocViews (6)
Cite

Package

Run in R for the authors' preferred citation:

citation("spiky")
APA
Triche, T., Harmon, L., & Wilson, S. (2026). spiky: Spike-in calibration for cell-free MeDIP (Version 1.18.0) [Computer software]. https://bioconductor.org/packages/spiky
About

This package has no citation file, so citation() returns this entry built from DESCRIPTION.

R Observatory

For a number measured here: a download total, a coverage figure, an archival date.

APA
Balamuta, J. J. (2026). R Observatory: Metrics for spiky version 1.18.0 [Data set]. HJJB, LLC. Data release v2026-10-26. https://doi.org/10.5281/zenodo.21843040
About

From data release v2026-10-26, which the citation names so these numbers can be found later.

Release 3.23: OK 22 Oct 2026

  • kunpeng2, Install

    Install on the build machine kunpeng2. Bioconductor release 3.23.

    OK

    Version built
    1.18.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    22 Oct 2026
  • kunpeng2, Build

    Build on the build machine kunpeng2. Bioconductor release 3.23.

    OK

    Version built
    1.18.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    22 Oct 2026
  • kunpeng2, Check

    Check on the build machine kunpeng2. Bioconductor release 3.23.

    OK

    Version built
    1.18.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    22 Oct 2026
  • nebbiolo1, Install

    Install on the build machine nebbiolo1. Bioconductor release 3.23.

    OK

    Version built
    1.18.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    22 Oct 2026
  • nebbiolo1, Build

    Build on the build machine nebbiolo1. Bioconductor release 3.23.

    OK

    Version built
    1.18.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    22 Oct 2026
  • nebbiolo1, Check

    Check on the build machine nebbiolo1. Bioconductor release 3.23.

    OK

    Version built
    1.18.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    22 Oct 2026
  • 6 OK
Machines (2)
MachineInstallBuildCheck
kunpeng2OKOKOK
nebbiolo1OKOKOK
Devel 3.24: Timeout
  • lconway, Check

    Check on the build machine lconway. The next Bioconductor release, 3.24.

    Timeout

    Version built
    1.19.0
    In this state since
    23 Oct 2026
    Last seen
    23 Oct 2026
  • lconway, Install

    Install on the build machine lconway. The next Bioconductor release, 3.24.

    OK

    Version built
    1.19.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    23 Oct 2026
  • lconway, Build

    Build on the build machine lconway. The next Bioconductor release, 3.24.

    OK

    Version built
    1.19.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    23 Oct 2026
  • lconway, Build binary

    Build binary on the build machine lconway. The next Bioconductor release, 3.24.

    OK

    Version built
    1.19.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    23 Oct 2026
  • nebbiolo2, Install

    Install on the build machine nebbiolo2. The next Bioconductor release, 3.24.

    OK

    Version built
    1.19.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    23 Oct 2026
  • nebbiolo2, Build

    Build on the build machine nebbiolo2. The next Bioconductor release, 3.24.

    OK

    Version built
    1.19.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    23 Oct 2026
  • nebbiolo2, Check

    Check on the build machine nebbiolo2. The next Bioconductor release, 3.24.

    OK

    Version built
    1.19.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    23 Oct 2026
  • taishan, Install

    Install on the build machine taishan. The next Bioconductor release, 3.24.

    OK

    Version built
    1.19.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    23 Oct 2026
  • taishan, Build

    Build on the build machine taishan. The next Bioconductor release, 3.24.

    OK

    Version built
    1.19.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    23 Oct 2026
  • taishan, Check

    Check on the build machine taishan. The next Bioconductor release, 3.24.

    OK

    Version built
    1.19.0
    In this state since
    29 Sep 2026 or earlier
    Last seen
    23 Oct 2026
  • 1 Timeout
  • 9 OK
MachineInstallBuildCheckBuild binary
lconwayOKOKTimeoutOK
nebbiolo2OKOKOK
taishanOKOKOK
Built files (4)
PlatformKindFile
macOS (Intel)binaryspiky_1.18.0.tgz
macOS (Apple Silicon)binaryspiky_1.18.0.tgz
sourcesourcespiky_1.18.0.tar.gz
Windowsbinaryspiky_1.18.0.zip
Total downloads in the latest complete month
4,055It comes from Bioconductor alone. Bioconda was counted over another window, so it is not added.

Rank in category, by distinct IPs

1,824 of 3,170

Bioconductor

4,055 downloads in the latest complete month

Distinct IP addresses average 514 a month over 12 months, which is a different measure and is never added.

It is in the total. Take it out

Bioconda

32 downloads in the last 30 days

It is not added because it has no figure for the latest complete month.

This local copy has no series over time.

Compare downloads

Functions
48
Public exports
34
Lines of R code
2,088
Median complexity
3
Functions (48)
Size and composition

Lines by kind (4,168)

KindLinesShare
R code2,08850%
Documentation1,69141%
Guides3899%

Lines by language

LanguageLinesShare
R2,08850%
Rd1,69141%
Vignettes2255%
Other1644%

Languages are counted from file extensions.

Files
119
R functions
48
Object systems
S4: 2 methods
Complexity
Median
3
Mean
3.38
Highest
12
Median lines per function
14.5
About complexity

Cyclomatic complexity counts the independent paths through a function: 1 is a straight line, higher is harder to test. R functions only.

Call graph figures
R functions
48
Calls
43
Unconnected
11
Groups
5
Growth (10 releases)
Total lines by release
3,587 on 26 Oct 20214,168 on 28 Apr 2026
Release changes

Test coverage is measured for CRAN packages only. Nothing is known for Bioconductor packages, which is not the same as no coverage.

Lines of tests
0
Exports named in tests
0%
Has tests
No
Test frameworks
testthat
Test lines per line of code
0
Exports documented
100%
Arguments documented
100%
Return value documented
100%
Help pages with examples
66.7%
Examples run in checks
96.7%
Guides (1)
Other documentation
README
Yes, 153 words
Release notes file
Yes
Help pages
45
Lines of documentation
1,691

12 datasets, 10 can be loaded by name

DatasetSizeColumns
2020_Human0.01_test100,000 × 6UMI, seq, rname, pos, X6547, X6548
2020_dedup_spikein_data26 × 6frag_grp, fragment_len, GC, CpG, read_count_6547, read_count_6548
dedupspike-in counts for two samples, as a wide data.frame26 × 3frag_grp, read_count_6547, read_count_6548
genbank_mitovarious mitochondrial genomes sometimes used as endogenous spike-ins
genomic_resA Granges object with genomic coverage from chr21q22, binned every 300bp for the genomic contigs then averaged across the bin. (In other words, the default output of scan_genomic_contigs or scan_genomic_bedpe, restricted to a small enough set of genomic regions to be practical for examples.) This re
phagelambda and phiX phage sequences, sometimes used as spike-ins
spikespike-in contig properties for Sam's cfMeDIP spikes
spike_cram_countsspike-in counts, as a long data.frame312 × 3frag_grp, id, read_count
spike_read_countsspike-in counts, as a long data.frame52 × 3frag_grp, id, read_count
spike_resA Granges object with spike-in sequence coverage, and summarized for each spike contig as (the default) `max` coverage. (In other words, the default output of scan_spike_contigs or scan_spike_bedpe) This represents what most users will want to generate from their own spike-in BAMs or BEDPEs, and is
ssb_resscan_spiked_bam results from a merged cfMeDIP CRAM file (chr22 and spikes)
testGRa test GRanges with UMI'ed genomic sequences used as controls

trichelab / spiky on github.com

Stars
3
Forks
6
Open issues
8
Last commit
16 Sep 2022
Activity
Commits
178
Open pull requests
0
Tagged releases
0
Latest tagged release
–
Issues closed in
68 days (median)
Pull requests closed in
0 days (median)
Pull requests merged
81%
Created
8 Sep 2020
Licence file reads as
–
Development practices (2)
KindFound
Continuous integrationTravis CI (config file)
Reproducibility and dev environmentMakefile

Method, repository practices

Across trichelab

Bioconductor git

R releases
  • In Bioconductor
  • Bioconductor release
ReleaseReleasedR
3.23 current29 Apr 20264.6
3.2230 Oct 20254.5
3.2116 Apr 20254.5
3.2030 Oct 20244.4
3.191 May 20244.4
3.1825 Oct 20234.3
3.1726 Apr 20234.3
3.162 Nov 20224.2
Earlier releases (2)
ReleaseReleasedR
3.1527 Apr 20224.2
3.1427 Oct 20214.1
Entered Bioconductor
Release 3.14, 27 Oct 2021