Genotype Calling with Uncertainty from Sequencing Data in Polyploids and Diploids
Overview
Read depth data from genotyping-by-sequencing (GBS) or restriction site-associated DNA sequencing (RAD-seq) are imported and used to make Bayesian probability estimates of genotypes in polyploids or diploids. The genotype probabilities, posterior mean genotypes, or most probable genotypes can then be exported for downstream analysis. 'polyRAD' is described by Clark et al. (2019) doi:10.1534/g3.118.200913, and the Hind/He statistic for marker filtering is described by Clark et al. (2022) doi:10.1186/s12859-022-04635-9. A variant calling pipeline for highly duplicated genomes is also included and is described by Clark et al. (2020, Version 1) doi:10.1101/2020.01.11.902890.
- Bug fix in
TestOverdispersionwhen the optimal value is the minimum tested. VCF2RADdatanow can make use of .csi indices on VCFs that are already bgzipped.- Bug fix in
Export_MAPpolyrelating to how ploidy is coded. - Bug fix in
AddAlleleFreqByTaxaif only one PC is used. - Update
readStacksfor new file formats. - Update Bioconductor links to pass CRAN checks
From NEWS.md in the release
Install
Health
NOTER code for possible problemsr-devel-linux-x86_64-debian-clang, r-devel-linux-x86_64-debian-gcc, r-devel-linux-x86_64-fedora-clang, r-devel-linux-x86_64-fedora-gcc, r-devel-windows-x86_64
Found calls to structure() using deprecated special names: polyRAD/inst/doc/isolocus_sorting.R (.Dim: 1, .Dimnames: 1) '.Dim' should be changed to 'dim'. '.Dimnames' should be changed to 'dimnames'.
- NOTE8 OK, 5 NOTENow
- NOTE10 OK, 3 NOTE2026-07-16
- OK12 OK2026-04-25
- NOTE11 OK, 3 NOTE2026-04-22
- ERROR10 OK, 3 NOTE, 1 ERROR2026-04-18
- NOTE11 OK, 3 NOTE2026-03-25
Show 1 earlier changeHide 1 earlier change
- WARNING8 OK, 3 NOTE, 3 WARNING2026-03-10
Documentation
- Help pages
- 49
- Examples that run
- 96%46 run in a plain check · 2 never run
- Exported functions with examples
- 98%
- Documented parameters
- 93%
- Return-value docs
- 100%
- Help pages citing references
- 29%
Downloads
Repository
- PRs merged (all time)
- 94%
- Median days to close, 50 most recently updated closed issues
- 29
- Median days to close, 50 most recently updated closed PRs
- 0
Stars over time
Forks over time
Releases over time
Issues over time
PRs over time
3 development-tooling and community-health practices detected across 2 families in the upstream repository
Checks run against github.com/lvclark/polyrad on 2026-10-18.
Dependencies
Installing polyRAD adds 3 packages (3 with compiled code, 13 MB of source tarballs). Heaviest: stringi 11 MB, Rcpp 1.8 MB, fastmatch 18 KB. 1 more not on CRAN is not measured.
Code & Tests
Datasets
People & History
Pick two releases to compare their code metrics.
- RR 4.6.0 released · 2026-04-24
- 2.0.1Latest
- RR 4.5.0 released · 2025-04-11
- RR 4.4.0 released · 2024-04-24
- RR 4.3.0 released · 2023-04-21
- 2.0.02022-11-06 · diff ↗ ·
removed 1 export
AddPriorTimesLikelihoodadded 4 exports
ExamineGenotype, GetTaxaByPloidy, GetTaxaPloidy, SetTaxaPloidy - RR 4.2.0 released · 2022-04-22
- 1.62022-02-15 · diff ↗
- 1.52021-09-03 · diff ↗ ·
added 4 exports
ExpectedHindHeMapping, Export_polymapR_probs, readDArTag, SimGenotypesMapping - RR 4.1.0 released · 2021-05-18
- 1.4
- 1.3
- 1.22020-05-12 · diff ↗ ·
added 9 exports
AddDepthSamplingPermutations, EstimateParentalGenotypes, HindHe, HindHeMapping, InbreedingFromHindHe, MergeIdenticalHaplotypes, RADdata2VCF, readProcessSamMulti, readProcessIsoloci - RR 4.0.0 released · 2020-04-24
- 1.12019-06-04 · diff ↗ ·
added 5 exports
Export_GWASpoly, Export_MAPpoly, MergeTaxaDepth, RemoveUngenotypedLoci, SubsetByPloidy - RR 3.6.0 released · 2019-04-26
Package metadata
- First published
- 2018-08-02
- Total releases
- 10 / 8 yrs
- License
- GPL (>= 2) OSI
- Minimum R
- ≥ 3.5.0
- Bundled data
- 173 KB / 3 files
- Download size
- 2.3 MB
Cite
Cite this package
Run in R for the authors' entry:
citation("polyRAD")The package provides a citation file, so citation() returns the authors' own entry.
It points to 10.1534/g3.118.200913, 10.1186/s12859-022-04635-9.
Cite the R Observatory
For a number measured here: a download total, a coverage figure, an archival date.
From data release v2026-10-20, which the citation names so these numbers can be found later. More on citing and the projects behind them.