Skip to content

SpotClean

Bioc current

SpotClean adjusts for spot swapping in spatial transcriptomics data

v1.14.0 · software · GPL-3

Release Lineage

Entered 3.16 · Nov 2, 2022

Current · Requires R 4.6

1.0 In 8 of 49 releases 3.23

Description

SpotClean is a computational method to adjust for spot swapping in spatial transcriptomics data. Recent spatial transcriptomics experiments utilize slides containing thousands of spots with spot-specific barcodes that bind mRNA. Ideally, unique molecular identifiers at a spot measure spot-specific expression, but this is often not the case due to bleed from nearby spots, an artifact we refer to as spot swapping. SpotClean is able to estimate the contamination rate in observed data and decontaminate the spot swapping effect, thus increase the sensitivity and precision of downstream analyses.

Test coverage

Line coverage

Expression

Tests / Examples

Functions

44 11 exported

Complexity

3 avg / 14 max

Call network

44 nodes / 28 edges

Test coverage is not measured for Bioconductor packages; nodes fall back to a neutral fill.

Loading call graph…

Lowest coverage

Per-function coverage is not measured for this package yet.

Code

Structure

Lines of code

3,588

Files

46

Compiled share

0%

Has compiled src

No

Language breakdown

R 2,106 (58.7%)Tests 452 (12.6%)Docs 775 (21.6%)Vignettes 255 (7.1%)

API

Exported functions

11

Internal functions

33

Testing & CI

Has tests

Yes

Test-to-code ratio

0.21

testthat edition

CI present

No

CI type

[]

PR gated

No

Docs

Roxygen coverage

100%

Health & Security signals

Informational signals; not verdicts.

on.exit coverage

Unsafe pattern score

0

Dep constraint coverage

0%

Secret pattern count

0

Bundled 3rd-party code

2 items

Portability & License

Min R version

4.2.0

System requirements

C++ standard

License

GPL-3

License flags

SPDX valid, OSI approved

History

Versions

8

First release

2022-11-22

Latest release

2026-04-28

Avg cadence

168 days

Cold removal rate

Dep drift

0

LOC over versions

v3.16: 3,556 LOCv3.17: 3,556 LOCv3.18: 3,586 LOCv3.19: 3,588 LOCv3.20: 3,588 LOCv3.21: 3,588 LOCv3.22: 3,588 LOCv3.23: 3,588 LOC

Per-file churn detail lives in the source pipeline: https://github.com/r-observatory/bioc-code-metrics.

Documentation

Documentation
READMEYes · 555 wordsVignettesYes · dynamicpkgdown siteNoNEWSYes · 67% structuredCode of conductNoContributing guideNo
Examples that run
100%
Documented parameters
100%
Return-value docs
100%
References docs
0%

Topics

People

Cite

Cite this package

Run in R for the authors' preferred citation:

citation("SpotClean")
Ni, Z., & Kendziorski, C. (2026). SpotClean: SpotClean adjusts for spot swapping in spatial transcriptomics data (Version 1.14.0) [Computer software]. https://bioconductor.org/packages/SpotClean

This is what citation() produces when a package has no citation file of its own. If it prints something else, use that.

Cite the R Observatory

For a number measured here: a download total, a coverage figure, an archival date.

APA

Balamuta, J. J. (2026). R Observatory: Metrics for SpotClean version 1.14.0 [Data set]. HJJB, LLC. Data release v2026-08-23. https://doi.org/10.5281/zenodo.21843040

From data release v2026-08-23, which the citation names so these numbers can be found later. More on citing and the projects behind them.

Report a problem with this page →

Privacy choices

These apply to this browser and are stored on this device only. Nothing about your choice is sent to us.

Read the privacy policy