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rtpcr

qPCR Data Analysis

v2.1.6 · Mar 14, 2026 · GPL-3

Description

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-test, Wilcoxon-test, ANOVA models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

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CRAN Check Status

3 ERROR
11 OK
Show all 14 flavors
Flavor Status
r-devel-linux-x86_64-debian-clang ERROR
r-devel-linux-x86_64-debian-gcc ERROR
r-devel-linux-x86_64-fedora-clang OK
r-devel-linux-x86_64-fedora-gcc OK
r-devel-macos-arm64 OK
r-devel-windows-x86_64 OK
r-oldrel-macos-arm64 OK
r-oldrel-macos-x86_64 OK
r-oldrel-windows-x86_64 OK
r-patched-linux-x86_64 OK
r-release-linux-x86_64 ERROR
r-release-macos-arm64 OK
r-release-macos-x86_64 OK
r-release-windows-x86_64 OK
Check details (3 non-OK)
ERROR r-devel-linux-x86_64-debian-clang

re-building of vignette outputs

Error(s) in re-building vignettes:
  ...
--- re-building ‘Method.Rmd’ using rmarkdown
pandoc: /usr/share/javascript/mathjax/MathJax.js: withBinaryFile: does not exist (No such file or directory)
HasCallStack backtrace:
  collectBacktraces, called at libraries/ghc-internal/src/GHC/Internal/Exception.hs:169:13 in ghc-internal:GHC.Internal.Exception
  toExceptionWithBacktrace, called at libraries/ghc-internal/src/GHC/Internal/IO.hs:260:11 in ghc-internal:GHC.Internal.IO
  throwIO, called at libraries/ghc-internal/src/GHC/Internal/IO/Exception.hs:315:19 in ghc-internal:GHC.Internal.IO.Exception
  ioException, called at libraries/ghc-internal/src/GHC/Internal/IO/Exception.hs:319:20 in ghc-internal:GHC.Internal.IO.Exception


Error: processing vignette 'Method.Rmd' failed with diagnostics:
pandoc document conversion failed with error 1
--- failed re-building ‘Method.Rmd’

--- re-building ‘manual.Rmd’ using rmarkdown
--- finished re-building ‘manual.Rmd’

SUMMARY: processing the following file failed:
  ‘Method.Rmd’

Error: Vignette re-building failed.
Execution halted
ERROR r-devel-linux-x86_64-debian-gcc

re-building of vignette outputs

Error(s) in re-building vignettes:
  ...
--- re-building ‘Method.Rmd’ using rmarkdown
pandoc: /usr/share/javascript/mathjax/MathJax.js: withBinaryFile: does not exist (No such file or directory)
HasCallStack backtrace:
  collectBacktraces, called at libraries/ghc-internal/src/GHC/Internal/Exception.hs:169:13 in ghc-internal:GHC.Internal.Exception
  toExceptionWithBacktrace, called at libraries/ghc-internal/src/GHC/Internal/IO.hs:260:11 in ghc-internal:GHC.Internal.IO
  throwIO, called at libraries/ghc-internal/src/GHC/Internal/IO/Exception.hs:315:19 in ghc-internal:GHC.Internal.IO.Exception
  ioException, called at libraries/ghc-internal/src/GHC/Internal/IO/Exception.hs:319:20 in ghc-internal:GHC.Internal.IO.Exception


Error: processing vignette 'Method.Rmd' failed with diagnostics:
pandoc document conversion failed with error 1
--- failed re-building ‘Method.Rmd’

--- re-building ‘manual.Rmd’ using rmarkdown
--- finished re-building ‘manual.Rmd’

SUMMARY: processing the following file failed:
  ‘Method.Rmd’

Error: Vignette re-building failed.
Execution halted
ERROR r-release-linux-x86_64

re-building of vignette outputs

Error(s) in re-building vignettes:
  ...
--- re-building ‘Method.Rmd’ using rmarkdown
pandoc: /usr/share/javascript/mathjax/MathJax.js: withBinaryFile: does not exist (No such file or directory)
HasCallStack backtrace:
  collectBacktraces, called at libraries/ghc-internal/src/GHC/Internal/Exception.hs:169:13 in ghc-internal:GHC.Internal.Exception
  toExceptionWithBacktrace, called at libraries/ghc-internal/src/GHC/Internal/IO.hs:260:11 in ghc-internal:GHC.Internal.IO
  throwIO, called at libraries/ghc-internal/src/GHC/Internal/IO/Exception.hs:315:19 in ghc-internal:GHC.Internal.IO.Exception
  ioException, called at libraries/ghc-internal/src/GHC/Internal/IO/Exception.hs:319:20 in ghc-internal:GHC.Internal.IO.Exception


Error: processing vignette 'Method.Rmd' failed with diagnostics:
pandoc document conversion failed with error 1
--- failed re-building ‘Method.Rmd’

--- re-building ‘manual.Rmd’ using rmarkdown
--- finished re-building ‘manual.Rmd’

SUMMARY: processing the following file failed:
  ‘Method.Rmd’

Error: Vignette re-building failed.
Execution halted

Check History

ERROR 13 OK · 0 NOTE · 0 WARNING · 1 ERROR · 0 FAILURE Apr 3, 2026
ERROR r-devel-linux-x86_64-debian-gcc

re-building of vignette outputs

Error(s) in re-building vignettes:
  ...
--- re-building ‘Method.Rmd’ using rmarkdown
pandoc: /usr/share/javascript/mathjax/MathJax.js: withBinaryFile: does not exist (No such file or directory)
HasCallStack backtrace:
  collectBacktraces, called at 
...[truncated]...
 with error 1
--- failed re-building ‘Method.Rmd’

--- re-building ‘manual.Rmd’ using rmarkdown
--- finished re-building ‘manual.Rmd’

SUMMARY: processing the following file failed:
  ‘Method.Rmd’

Error: Vignette re-building failed.
Execution halted
OK 14 OK · 0 NOTE · 0 WARNING · 0 ERROR · 0 FAILURE Mar 10, 2026

Dependency Network

Dependencies Reverse dependencies multcomp ggplot2 lme4 lmerTest purrr reshape2 tidyr dplyr emmeans lifecycle rtpcr

Version History

updated 2.1.6 ← 2.1.5 diff Mar 14, 2026
new 2.1.5 Mar 10, 2026
updated 2.1.5 ← 2.1.4 diff Feb 23, 2026
updated 2.1.4 ← 2.1.3 diff Feb 11, 2026
updated 2.1.3 ← 2.1.2 diff Feb 2, 2026
updated 2.1.2 ← 2.1.1 diff Jan 22, 2026
updated 2.1.1 ← 2.1.0 diff Jan 7, 2026
updated 2.1.0 ← 2.0.5 diff Dec 18, 2025
updated 2.0.5 ← 2.0.4 diff Dec 11, 2025
updated 2.0.4 ← 2.0.3 diff Dec 3, 2025
updated 2.0.3 ← 2.0.2 diff Nov 7, 2025
updated 2.0.2 ← 2.0.1 diff Mar 24, 2025
updated 2.0.1 ← 2.0.0 diff Feb 12, 2025
updated 2.0.0 ← 1.0.9 diff Jul 6, 2024
updated 1.0.9 ← 1.0.8 diff May 29, 2024
updated 1.0.8 ← 1.0.7 diff May 15, 2024
updated 1.0.7 ← 1.0.6 diff May 9, 2024
updated 1.0.6 ← 1.0.5 diff Apr 28, 2024
updated 1.0.5 ← 1.0.4 diff Apr 20, 2024
updated 1.0.4 ← 1.0.3 diff Apr 19, 2024