qPCR Ct Values to Expression Values
Overview
Computes normalized cycle threshold (Ct) values (delta Ct) from raw quantitative polymerase chain reaction (qPCR) Ct values and conducts test of significance using t.test(). Plots expression values based from log2(2^(-1*delta delta Ct)) across groups per gene of interest. Methods for calculation of delta delta Ct and relative expression (2^(-1*delta delta Ct)) values are described in: Livak & Schmittgen, (2001) doi:10.1006/meth.2001.1262.
Notes:
- I hope everyone who comes across this tool will find value in it! Enjoy!
s
From NEWS in the release
Install
Health
NOTECRAN incoming feasibilityr-devel-linux-x86_64-debian-clang, r-devel-linux-x86_64-debian-gcc
Maintainer: ‘Wilson Jr. Aala <s98107020@gs.ncku.edu.tw>’
No Authors@R field in DESCRIPTION.
Please add one, modifying
Authors@R: person(given = c("Wilson", "Jr."),
family = "Aala",
role = c("aut", "cre"),
email = "s98107020@gs.ncku.edu.tw")
as necessary.- NOTE11 OK, 2 NOTE2026-06-09
- ERROR11 OK, 1 NOTE, 1 ERROR2026-06-08
- NOTE12 OK, 2 NOTE2026-03-10
Documentation
- Help pages
- 1
- Examples that run
- 100%1 run in a plain check
- Exported functions with examples
- 100%
- Documented parameters
- not tracked
- Return-value docs
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- Help pages citing references
- 0%
- qPCRhelper HTML
Downloads
Dependencies
Code & Tests
People & History
- RR 4.6.0 released · 2026-04-24
- RR 4.5.0 released · 2025-04-11
- RR 4.4.0 released · 2024-04-24
- RR 4.3.0 released · 2023-04-21
- 0.1.0Latest2023-02-23 · current release
- RR 4.2.0 released · 2022-04-22
Package metadata
- First published
- 2023-02-23
- Total releases
- 1 / 3 yrs
- License
- GPL-3 OSI
- Download size
- 8.2 KB
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