Analyze High-Dimensional High-Throughput Dataset and Quality Control Single-Cell RNA-Seq
Overview
The advent of genomic technologies has enabled the generation of two-dimensional or even multi-dimensional high-throughput data, e.g., monitoring multiple changes in gene expression in genome-wide siRNA screens across many different cell types (E Robert McDonald 3rd (2017) <doi: 10.1016/j.cell.2017.07.005> and Tsherniak A (2017) <doi: 10.1016/j.cell.2017.06.010>) or single cell transcriptomics under different experimental conditions. We found that simple computational methods based on a single statistical criterion is no longer adequate for analyzing such multi-dimensional data. We herein introduce 'ZetaSuite', a statistical package initially designed to score hits from two-dimensional RNAi screens.We also illustrate a unique utility of 'ZetaSuite' in analyzing single cell transcriptomics to differentiate rare cells from damaged ones (Vento-Tormo R (2018) <doi: 10.1038/s41586-018-0698-6>). In 'ZetaSuite', we have the following steps: QC of input datasets, normalization using Z-transformation, Zeta score calculation and hits selection based on defined Screen Strength.
Install
Health
- OK13 OK2026-08-05
- NOTE12 OK, 1 NOTE2026-08-01
- OK13 OK2026-06-09
- ERROR12 OK, 1 ERROR2026-06-08
- OK14 OK2026-03-10
Documentation
- Help pages
- 15
- Examples that run
- 93%14 run in a plain check · 1 never run
- Exported functions with examples
- 100%
- Documented parameters
- 100%
- Return-value docs
- 100%
- Help pages citing references
- 7%
- ZetaSuite HTML
Downloads
Repository
Checks run against github.com/junhuili1017/zetasuite on 2026-10-21.
No development-tooling practices detected in the upstream repository.
Dependencies
Installing ZetaSuite adds 81 packages (45 with compiled code, 74 MB of source tarballs). Heaviest: stringi 11 MB, ggplot2 6.0 MB, data.table 5.7 MB.
Code & Tests
Datasets
People & History
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- 1.0.3Latest
- RR 4.6.0 released · 2026-04-24
- 1.0.2
- RR 4.5.0 released · 2025-04-11
- RR 4.4.0 released · 2024-04-24
- RR 4.3.0 released · 2023-04-21
- 1.0.12022-05-24 · diff ↗
- RR 4.2.0 released · 2022-04-22
- 1.0.02022-03-31
- RR 4.1.0 released · 2021-05-18
Package metadata
- First published
- 2022-03-31
- Total releases
- 4 / 4 yrs
- License
- MIT + file LICENSE OSI · relicensed from GPL-2 | GPL-3 in 1.0.2
- Minimum R
- ≥ 2.10
- Bundled data
- 3.5 MB / 8 files
- Download size
- 4.2 MB
Cite
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