Relative Quantification of Gene Expression using Delta Ct Methods
Overview
The commonly used methods for relative quantification of gene expression levels obtained in real-time PCR (Polymerase Chain Reaction) experiments are the delta Ct methods, encompassing 2^-dCt and 2^-ddCt methods, originally proposed by Kenneth J. Livak and Thomas D. Schmittgen (2001) doi:10.1006/meth.2001.1262. The main idea is to normalise gene expression values using endogenous control gene, present gene expression levels in linear form by using the 2^-(value)^ transformation, and calculate differences in gene expression levels between groups of samples (or technical replicates of a single sample). The 'RQdeltaCT' package offers functions that cover both methods for comparison of either independent groups of samples or groups with paired samples, together with importing expression datasets, performing multi-step quality control of data, enabling numerous data visualisations, enrichment of the standard workflow with additional useful analyses (correlation analysis, Receiver Operating Characteristic analysis, logistic regression), and conveniently export obtained results in table and image formats. The package has been designed to be friendly to non-experts in R programming.
Install
Health
- OK13 OK2026-09-09
- ERROR12 OK, 1 ERROR2026-09-04
- OK13 OK2026-08-05
- NOTE12 OK, 1 NOTE2026-08-01
- OK13 OK2026-06-09
Show 2 earlier changesHide 2 earlier changes
- ERROR12 OK, 1 ERROR2026-06-08
- OK14 OK2026-03-10
Documentation
- Help pages
- 36
- Examples that run
- 100%32 run in a plain check
- Exported functions with examples
- 100%
- Documented parameters
- 94%
- Return-value docs
- 100%
- Help pages citing references
- 0%
Downloads
Repository
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Issues over time
1 development-tooling and community-health practice detected across 1 family in the upstream repository
Checks run against github.com/donadelnal/rqdeltact on 2026-10-18.
Dependencies
Code & Tests
Datasets
People & History
Pick two releases to compare their code metrics.
- RR 4.6.0 released · 2026-04-24
- 1.3.3Latest
- RR 4.5.0 released · 2025-04-11
- 1.3.2
- RR 4.4.0 released · 2024-04-24
- 1.3.02024-04-17 · diff ↗ ·
removed 4 exports
RQ_exp_Ct_dCt, RQ_plot, RQ_volcano, exp_Ct_dCtadded 3 exports
FCh_plot, RQ_dCt, results_volcano - 1.2.12024-04-02
- RR 4.3.0 released · 2023-04-21
Package metadata
- First published
- 2024-04-02
- Total releases
- 4 / 2 yrs
- License
- GPL-3 OSI
- Minimum R
- ≥ 2.10
- Bundled data
- 13 KB / 4 files
- Download size
- 3.4 MB
Cite
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Run in R for the authors' entry:
citation("RQdeltaCT")The package provides a citation file, so citation() returns the authors' own entry.
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