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VALERIE

Visualising Splicing at Single-Cell Resolution

v1.1.0 · Jul 10, 2020 · GPL-3

Description

Alternative splicing produces a variety of different protein products from a given gene. 'VALERIE' enables visualisation of alternative splicing events from high-throughput single-cell RNA-sequencing experiments. 'VALERIE' computes percent spliced-in (PSI) values for user-specified genomic coordinates corresponding to alternative splicing events. PSI is the proportion of sequencing reads supporting the included exon/intron as defined by Shiozawa (2018) <doi:10.1038/s41467-018-06063-x>. PSI are inferred from sequencing reads data based on specialised infrastructures for representing and computing annotated genomic ranges by Lawrence (2013) <doi:10.1371/journal.pcbi.1003118>. Computed PSI for each single cell are subsequently presented in the form of a heatmap implemented using the 'pheatmap' package by Kolde (2010) <https://CRAN.R-project.org/package=pheatmap>. Board overview of the mean PSI difference and associated p-values across different user-defined groups of single cells are presented in the form of a line graph using the 'ggplot2' package by Wickham (2007) <https://CRAN.R-project.org/package=ggplot2>.

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CRAN Check Status

1 ERROR
4 NOTE
9 OK
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Flavor Status
r-devel-linux-x86_64-debian-clang NOTE
r-devel-linux-x86_64-debian-gcc NOTE
r-devel-linux-x86_64-fedora-clang OK
r-devel-linux-x86_64-fedora-gcc OK
r-devel-macos-arm64 OK
r-devel-windows-x86_64 OK
r-oldrel-macos-arm64 NOTE
r-oldrel-macos-x86_64 NOTE
r-oldrel-windows-x86_64 ERROR
r-patched-linux-x86_64 OK
r-release-linux-x86_64 OK
r-release-macos-arm64 OK
r-release-macos-x86_64 OK
r-release-windows-x86_64 OK
Check details (6 non-OK)
NOTE r-devel-linux-x86_64-debian-clang

CRAN incoming feasibility

Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’

No Authors@R field in DESCRIPTION.
Please add one, modifying
  Authors@R: person(given = "Sean",
                    family = "Wen",
                    role = c("aut", "cre"),
                    email = "sean.wenwx@gmail.com")
as necessary.
NOTE r-devel-linux-x86_64-debian-gcc

CRAN incoming feasibility

Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’

No Authors@R field in DESCRIPTION.
Please add one, modifying
  Authors@R: person(given = "Sean",
                    family = "Wen",
                    role = c("aut", "cre"),
                    email = "sean.wenwx@gmail.com")
as necessary.
NOTE r-oldrel-macos-arm64

installed package size

installed size is  9.4Mb
  sub-directories of 1Mb or more:
    extdata   8.7Mb
NOTE r-oldrel-macos-x86_64

installed package size

installed size is  9.4Mb
  sub-directories of 1Mb or more:
    extdata   8.7Mb
NOTE r-oldrel-windows-x86_64

installed package size

installed size is  9.5Mb
  sub-directories of 1Mb or more:
    extdata   8.8Mb
ERROR r-oldrel-windows-x86_64

re-building of vignette outputs

Error(s) in re-building vignettes:
--- re-building 'VALERIE.Rmd' using rmarkdown
! Package pdftex.def Error: File `ae1f81e7f5d6239e8c4e5d45bf832245c4cad9ee.pdf'
 not found: using draft setting.

Error: processing vignette 'VALERIE.Rmd' failed with diagnostics:
LaTeX failed to compile D:/RCompile/CRANpkg/local/4.4/VALERIE.Rcheck/vign_test/VALERIE/vignettes/VALERIE.tex. See https://yihui.org/tinytex/r/#debugging for debugging tips. See VALERIE.log for more info.
--- failed re-building 'VALERIE.Rmd'

SUMMARY: processing the following file failed:
  'VALERIE.Rmd'

Error: Vignette re-building failed.
Execution halted

Check History

ERROR 9 OK · 4 NOTE · 0 WARNING · 1 ERROR · 0 FAILURE Mar 30, 2026
NOTE r-devel-linux-x86_64-debian-clang

CRAN incoming feasibility

Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’

No Authors@R field in DESCRIPTION.
Please add one, modifying
  Authors@R: person(given = "Sean",
                    family = "Wen",
                    role = c("aut", "cre"),
                    email = "sean.wenwx@gmail.com")
as necessary.
NOTE r-devel-linux-x86_64-debian-gcc

CRAN incoming feasibility

Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’

No Authors@R field in DESCRIPTION.
Please add one, modifying
  Authors@R: person(given = "Sean",
                    family = "Wen",
                    role = c("aut", "cre"),
                    email = "sean.wenwx@gmail.com")
as necessary.
NOTE r-oldrel-macos-arm64

installed package size

installed size is  9.4Mb
  sub-directories of 1Mb or more:
    extdata   8.7Mb
NOTE r-oldrel-macos-x86_64

installed package size

installed size is  9.4Mb
  sub-directories of 1Mb or more:
    extdata   8.7Mb
ERROR r-oldrel-windows-x86_64

installed package size

installed size is  9.5Mb
  sub-directories of 1Mb or more:
    extdata   8.8Mb
NOTE 9 OK · 5 NOTE · 0 WARNING · 0 ERROR · 0 FAILURE Mar 10, 2026
NOTE r-devel-linux-x86_64-debian-clang

CRAN incoming feasibility

Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’

No Authors@R field in DESCRIPTION.
Please add one, modifying
  Authors@R: person(given = "Sean",
                    family = "Wen",
                    role = c("aut", "cre"),
                    email = "sean.wenwx@gmail.com")
as necessary.
NOTE r-devel-linux-x86_64-debian-gcc

CRAN incoming feasibility

Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’

No Authors@R field in DESCRIPTION.
Please add one, modifying
  Authors@R: person(given = "Sean",
                    family = "Wen",
                    role = c("aut", "cre"),
                    email = "sean.wenwx@gmail.com")
as necessary.
NOTE r-oldrel-macos-arm64

installed package size

installed size is  9.4Mb
  sub-directories of 1Mb or more:
    extdata   8.7Mb
NOTE r-oldrel-macos-x86_64

installed package size

installed size is  9.4Mb
  sub-directories of 1Mb or more:
    extdata   8.7Mb
NOTE r-oldrel-windows-x86_64

installed package size

installed size is  9.5Mb
  sub-directories of 1Mb or more:
    extdata   8.8Mb

Dependency Network

Dependencies Reverse dependencies GenomicAlignments GenomicRanges IRanges Rsamtools plyr ggplot2 pheatmap ggplotify ggpubr scales VALERIE

Version History

new 1.1.0 Mar 10, 2026
updated 1.1.0 ← 1.0.0 diff Jul 9, 2020
new 1.0.0 Feb 10, 2020